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Overview: [membrane
organization] [overview
of the study ] [research
paper]
Part 1:
[components
of blood] [blood
and red cell fractionation]
Part 2: [introduction
to SDS-PAGE] [preparing
gels] [preparing
samples] [assembly,
loading, running gels]
Part 3: [gel
images] [gel
analysis] [molecular
mass standard curve] [measuring
relative mobility] ["Hall
of Shame"]
Methods: [tissue
fractionation] [centrifugation]
[Bradford protein
assay]
 Individual Gel PhotosPlease find your name, open the link to your gel photo, and print off two copies to bring to the laboratory for analysis. You will also need copies of the other gel on which your samples were loaded, so we hope that you noted the names of your partner group. One copy of each image is for the analysis, on which you may wish to write notes and otherwise mark up. You will need clean copies to be labeled and submitted with your results section. Your partners may need one or two copies of your gel photo in case they forgot who you are and couldn't print your picture. PRINTING: Each figure is a JPEG document that you may capture and insert into a text or other type file, or simply print off. For the final results section you should at least try to insert the image into a text file. It will not be necessary to print off the gels in color. You are welcome to make modifications to an image such as improving brightness and/or contrast, but any enhancements must apply to the entire image. You must not selectively intensify specific bands, for example. If you clearly have a bad gel you may search for better examples for analysis and print off appropriate images. You will have to provide a critique of your own gels in the results section of your paper, but you may use a better gel for the characterization. Here are links to two good ones, both from this semester. The loading pattern is the pattern we recommended in class. Alphabetical list (Friday section will be set up some time Monday 28-Jan)Alarcon, Peter |
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Created by David R. Caprette (caprette@rice.edu), Rice University9 Feb 04
Updated 26 Jan 08