BIOE342
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BIOE342 Tissue Culture Laboratory

SECTION 001 SCHEDULE

Lab Time: Mon 1pm-6pm, Wed 1pm-6pm, Fri one hour during afternoon
Lab Sessions: Jan 14, 16, 21, 23, 26, 28, 30, Feb 2, 4, 6, 9, 11, 13, 16, 18
Lecture: Fri 2-2:50 pm, Jan 16, 23, 30, Feb 6, 13

  IntroductoryMaterial Cell Survival and Function in vitro GFP Transfection Assay
Session 1
(W, 14 Jan)
  • Micropipette
  • Cell Visualization
  • Sterile technique
  • Contamination
  • pH and color
    		•    
    Session 2
    (W, 21 Jan)
  • cell concentration
  • sterile technique
  • contamination
    		•     
    Session 3
    (M, 26 Jan)    		  
  • Fn attatchment
  • MTT prep
  • Live/dead staining prep
    		•   
    Session 4
    (W, 28 Jan)    		  
  • MTT Assay
  • live / dead staining
    		•   
    Session 5
    (M, 2 Feb)    		  
  • attatchment time
  • PCNA staining prep
    		•   
    Session 6
    (W, 4 Feb)    		  
  • proliferation
  • PCNA staining
    		•   
    Session 7
    (M, 9 Feb)    		  
  • proliferation
    		•
  • GFP expt
    		•
    Session 8
    (W, 11 Feb)    		  
  • proliferation
    		•
  • GFP expt
    		•
    Session 9
    (M, 16 Feb)    		    
  • GFP expt
    		•
    Session 10
    (W, 18 Feb)    		    
  • GFP expt
    		•

    Session 1 (M)
  • Micropipette exercise.
  • Visualization of cells.
  • Practice sterile technique - Session 1.
  • Contamination exercise.
  • pH and color of media.


    • Session 2 (W)
  • Look at agar plates from contamination exercise.
  • Determination of cell concentration.
  • Practice sterile technique - Session 2.
  • Each student to get a flask of cells.

    Session 3 (M)
  • Fn attachment assay.
  • MTT assay (Day 1).
  • Live/dead assay (Day 1).
  • Feed and/or passage flask of cells.


    • Session 4 (W)
  • MTT assay (Day 3).
  • Live/dead assay (Day 3).
  • Feed and/or passage flask of cells.


    • Session 5 (M)
  • Attachment assay.
  • PCNA staining assay (Day 1).
  • Feed and/or passage flask of cells.

    Session 6 (W)
  • Cell proliferation assay (Day 1).
  • PCNA staining assay (Day 3).
  • Feed and/or passage flask of cells.


    • Friday after Session 6 (F)
  • Cell proliferation assay (Day 3).


    • Session 7 (M)
  • GFP transfection assay (Day 1).
  • Cell proliferation assay (Day 6).
  • Feed and/or passage flask of cells.


    • Session 8 (W)
  • GFP transfection assay (Day 3).
  • Cell proliferation assay (Day 8).
  • Feed and/or passage flask of cells.


    • Friday after Session 8 (F)
  • GFP transfection assay (Day 5).


    • Session 9 (M)
  • GFP transfection assay (Day 8).


    • Session 10 (W)
  • GFP transfection assay (Day 10).

    • On Fridays, cells need to be fed and/or passaged.


    SECTION 002 SCHEDULE

    Lab Time: Tues 1pm-6pm, Thurs 1pm-6pm, Sat one hour (time TBA)
    Lab Sessions: Jan 20, 22, 24, 27, 29, 31, Feb 3, 5, 7, 10, 12, 14, 17, 19
    Lecture: Fri 2-2:50 pm, Jan 16, 23, 30, Feb 6, 13


      IntroductoryMaterial Cell Survival and Function in vitro GFP Transfection Assay
    Session 1
    (Tu, 20 Jan)
  • Micropipette
  • Cell Visualization
  • Sterile technique
  • Contamination
  • pH and color
    		•    
    Session 2
    (Th, 22 Jan)
  • cell concentration
  • sterile technique
  • contamination
    		•     
    Session 3
    (Tu, 27 Jan)    		  
  • Fn attatchment
  • MTT prep
  • Live/dead staining prep
    		•   
    Session 4
    (Th, 29 Jan)    		  
  • MTT Assay
  • live / dead staining
    		•   
    Session 5
    (Tu, 3 Feb)    		  
  • attatchment time
  • PCNA staining prep
    		•   
    Session 6
    (Th, 5 Feb)    		  
  • proliferation
  • PCNA staining
    		•   
    Session 7
    (Tu, 10 Feb)    		  
  • proliferation
    		•
  • GFP expt
    		•
    Session 8
    (Th, 12 Feb)     		 
  • proliferation
    		•
  • GFP expt
    		•
    Session 9
    (Tu, 17 Feb)    		    
  • GFP expt
    		•
    Session 10
    (Th, 19 Feb)    		    
  • GFP expt
    		•

    Session 1 (Tu)
  • Micropipette exercise.
  • Visualization of cells.
  • Practice sterile technique - Session 1.
  • Contamination exercise.
  • pH and color of media.


    • Session 2 (Th)
  • Look at agar plates from contamination exercise
  • Determination of cell concentration.
  • Practice sterile technique - Session 2.
  • Each student to get a flask of cells.
  • Look at agar plates from contamination exercise.


    • Session 3 (Tu)
  • Fn attachment assay.
  • MTT assay (Day 1).
  • Live/dead assay (Day 1).
  • Feed and/or passage flask of cells.


    • Session 4 (Th)
  • MTT assay (Day 3).
  • Live/dead assay (Day 3).
  • Feed and/or passage flask of cells.


    • Session 5 (Tu)
  • Attachment assay.
  • PCNA staining assay (Day 1).
  • Feed and/or passage flask of cells.

    Session 6 (Th)
  • Cell proliferation assay (Day 1).
  • PCNA staining assay (Day 3).
  • Feed and/or passage flask of cells.


    • Saturday after Session 6 (Sa)
  • Cell proliferation assay (Day 3).


    • Session 7 (Tu)
  • GFP transfection assay (Day 1).
  • Cell proliferation assay (Day 6).
  • Feed and/or passage flask of cells.


    • Session 8 (Th)
  • GFP transfection assay (Day 3).
  • Cell proliferation assay (Day 8).
  • Feed and/or passage flask of cells.


    • Saturday after Session 8 (Sa)
  • GFP transfection assay (Day 5).


    • Session 9 (Tu)
  • GFP transfection assay (Day 8).


    • Session 10 (Th)
  • GFP transfection assay (Day 10).

    • On Saturdays, cells need to be fed and/or passaged.


    SECTION 003 SCHEDULE

    Lab Time: Mon 1pm-6pm, Wed 1pm-6pm, Fri one hour during afternoon
    Lab Sessions: Mar 8, 10, 12, 15, 17, 19, 22, 24, 26, 29, 31, April 2, 5, 7
    Lecture: Fri 2-2:50 pm, Mar 12, 19, 26, April 2

      IntroductoryMaterial Cell Survival and Function in vitro GFP Transfection Assay
    Session 1
    (M, 8 Mar)
  • Micropipette
  • Cell Visualization
  • Sterile technique
  • Contamination
  • pH and color
    		•    
    Session 2
    (W, 10 Mar)
  • cell concentration
  • sterile technique
  • contamination
    		•     
    Session 3
    (M, 15 Mar)    		  
  • Fn attatchment
  • MTT prep
  • Live/dead staining prep
    		•   
    Session 4
    (W, 17 Mar)    		  
  • MTT Assay
  • live / dead staining
    		•   
    Session 5
    (M, 22 Mar)    		  
  • attatchment time
  • PCNA staining prep
    		•   
    Session 6
    (W, 24 Mar)    		  
  • proliferation
  • PCNA staining
    		•   
    Session 7
    (M, 29 Mar)    		  
  • proliferation
    		•
  • GFP expt
    		•
    Session 8
    (W, 31 Mar)     		 
  • proliferation
    		•
  • GFP expt
    		•
    Session 9
    (M, 5 Apr)    		    
  • GFP expt
    		•
    Session 10
    (W, 7 Apr)    		    
  • GFP expt
    		•

    Session 1 (M)
  • Micropipette exercise.
  • Visualization of cells.
  • Practice sterile technique - Session 1.
  • Contamination exercise.
  • pH and color of media.


    • Session 2 (W)
  • Look at agar plates from contamination exercise.
  • Determination of cell concentration.
  • Practice sterile technique - Session 2.
  • Each student to get a flask of cells.

    Session 3 (M)
  • Fn attachment assay.
  • MTT assay (Day 1).
  • Live/dead assay (Day 1).
  • Feed and/or passage flask of cells.


    • Session 4 (W)
  • MTT assay (Day 3).
  • Live/dead assay (Day 3).
  • Feed and/or passage flask of cells.


    • Session 5 (M)
  • Attachment assay.
  • PCNA staining assay (Day 1).
  • Feed and/or passage flask of cells.

    Session 6 (W)
  • Cell proliferation assay (Day 1).
  • PCNA staining assay (Day 3).
  • Feed and/or passage flask of cells.


    • Friday after Session 6 (F)
  • Cell proliferation assay (Day 3).


    • Session 7 (M)
  • GFP transfection assay (Day 1).
  • Cell proliferation assay (Day 6).
  • Feed and/or passage flask of cells.


    • Session 8 (W)
  • GFP transfection assay (Day 3).
  • Cell proliferation assay (Day 8).
  • Feed and/or passage flask of cells.


    • Friday after Session 8 (F)
  • GFP transfection assay (Day 5).


    • Session 9 (M)
  • GFP transfection assay (Day 8).


    • Session 10 (W)
  • GFP transfection assay (Day 10).

    • On Fridays, cells need to be fed and/or passaged.


    SECTION 004 SCHEDULE

    Lab Time: Tues 1pm-6pm, Thurs 1pm-6pm, Sat one hour (time TBA)
    Lab Sessions: Mar 9, 11, 13, 16, 18, 20, 23, 25, 27, 30, April 1, 3, 6, 8
    Lecture: Fri 2-2:50 pm, Mar 12, 19, 26, April 2


      IntroductoryMaterial Cell Survival and Function in vitro GFP Transfection Assay
    Session 1
    (Tu, 9 Mar)
  • Micropipette
  • Cell Visualization
  • Sterile technique
  • Contamination
  • pH and color
    		•    
    Session 2
    (Th, 11 Mar)
  • cell concentration
  • sterile technique
  • contamination
    		•     
    Session 3
    (Tu, 16 Mar)    		  
  • Fn attatchment
  • MTT prep
  • Live/dead staining prep
    		•   
    Session 4
    (Th, 18 Mar)    		  
  • MTT Assay
  • live / dead staining
    		•   
    Session 5
    (Tu, 23 Mar)    		  
  • attatchment time
  • PCNA staining prep
    		•   
    Session 6
    (Th, 25 Mar)    		  
  • proliferation
  • PCNA staining
    		•   
    Session 7
    (Tu, 30 Mar)    		  
  • proliferation
    		•
  • GFP expt
    		•
    Session 8
    (Th, 1 Apr)     		 
  • proliferation
    		•
  • GFP expt
    		•
    Session 9
    (Tu, 6 Apr)    		    
  • GFP expt
    		•
    Session 10
    (Th, 8 Apr)    		    
  • GFP expt
    		•

    Session 1 (Tu)
  • Micropipette exercise.
  • Visualization of cells.
  • Practice sterile technique - Session 1.
  • Contamination exercise.
  • pH and color of media.


    • Session 2 (Th)
  • Look at agar plates from contamination exercise
  • Determination of cell concentration.
  • Practice sterile technique - Session 2.
  • Each student to get a flask of cells.
  • Look at agar plates from contamination exercise.


    • Session 3 (Tu)
  • Fn attachment assay.
  • MTT assay (Day 1).
  • Live/dead assay (Day 1).
  • Feed and/or passage flask of cells.


    • Session 4 (Th)
  • MTT assay (Day 3).
  • Live/dead assay (Day 3).
  • Feed and/or passage flask of cells.


    • Session 5 (Tu)
  • Attachment assay.
  • PCNA staining assay (Day 1).
  • Feed and/or passage flask of cells.

    Session 6 (Th)
  • Cell proliferation assay (Day 1).
  • PCNA staining assay (Day 3).
  • Feed and/or passage flask of cells.


    • Saturday after Session 6 (Sa)
  • Cell proliferation assay (Day 3).


    • Session 7 (Tu)
  • GFP transfection assay (Day 1).
  • Cell proliferation assay (Day 6).
  • Feed and/or passage flask of cells.


    • Session 8 (Th)
  • GFP transfection assay (Day 3).
  • Cell proliferation assay (Day 8).
  • Feed and/or passage flask of cells.


    • Saturday after Session 8 (Sa)
  • GFP transfection assay (Day 5).


    • Session 9 (Tu)
  • GFP transfection assay (Day 8).


    • Session 10 (Th)
  • GFP transfection assay (Day 10).

    • On Saturdays, cells need to be fed and/or passaged.

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