BIOE342 - Tissue Culture Laboratory
BIOE442 - Tissue Engineering Laboratory


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BIOE342 Tissue Culture Laboratory


Poster of HDF Cell Experiments
A majority of the experiments will focus in the Survival and Function of HDF (human dermal fibroblast) cells in vitro. Each student is required to put together a poster that covers the objectives, succinct summary of experimental methods, experimental results, and implications of these experiments.
A draft of the poster is due at the beginning of lecture (2:00 p.m.) on Friday, February 20. The posters will be critiqued during the lecture time that day, graded, and returned by the following Monday. A revised/final poster is due Friday, February 27 at 4:30 p.m. The poster must be turned into the BIOE Department Office (Keck 116).
Further information on the format and content of the poster will be distributed at a later date. Dr. Tracy Volz (Cain Foundation) will be a resource for this activity. The poster on the HDF Cell Experiments will count 35% of your final grade (15% draft; 20% revised/final).

Lab Notebook Maintenance
Carbon copy lab notebooks must be used. Copies of the work from Monday, Wednesday, and Friday of each week must be detached from your notebook and turned in on the following Monday at 1:00 p.m. For example, the writing in your lab notebook from Jan. 26, 28, and 30 should be turned on Feb. 2. Staple your pages together.
BIOE 342 will use the same format for keeping a lab notebook as BIOS 311; instructions and information can be found on the web at
bios311/notebook.html. However, there are two differences. First, graphs, charts, and plots (raw data and analyzed data) must be placed (stapled or taped) in your lab notebook (on original and carbon copy pages); a separate binder with charts should not be created. Second, you do not need to copy down all the protocols from the protocol book if you followed them exactly. You can note: The Micropipette Exercise was followed as given on pages 7-8 of the BIOE 342 Lab Protocols Book. However, when you make changes from the protocol, they should be noted. In addition, the cells type and passage number should be noted for all experiments.
A simple grading scale of Ö+ (A), Ö (B), and Ö- (C) will be used. Maintaining your lab notebook will count 15% in your final grade.

Experiment and Analysis Questions
Listed at the end of most protocols are Experimental and Analysis Questions. Answers to Questions must be written in your lab notebook. Copies of the work from Monday, Wednesday, and Friday of each week must be detached from your notebook and turned in on the following Monday at 1:00 p.m. (See example above.) Staple your pages together.
Questions must be answered once the last day of a particular protocol has been completed. For example, if an experiment ran Monday and Wednesday, the Questions must be answered and turned in by the following Monday.
Please number your answers to Questions. If possible, keep your answers to Questions for a particular experiment together and near that experiment. Answers to Questions will count 30% in your final grade.

All students are expected to work safely in the lab. Safety glasses must be worn at all times. Contacts may not be worn. Students must wear long pants, shirts with shoulders (or a lab coat, buttoned up), and close-toed shoes. Students not adhering to these policies will be sent home to change. Absolutely no eating, drinking or gum chewing will be allowed at any place or time in the lab (including tables in Teaching Lab).
Biohazardous material must be disposed of properly. Details for proper disposal will be discussed in lecture.
Adherence to safety policies will count 5% of your final grade. However, the instructor retains the right to penalize a student's final grade if egregious safety violations are noted.

Developing Tissue Culture Skills
A major objective of this course is to learn cell and tissue culture. The instructor and T.A.s will be working with and observing students one-on-one. Frequent verbal and written (minimum of three times) feedback will be given to students. A simple grading scale of Ö+ (A), Ö (B), and Ö- (C) will be used. Evaluations of Tissue Culture Skills will count 15% in your final grade.

In an ongoing effort to restructure science and engineering labs, self-evaluations must be completed at the beginning and end of this course. The self-evaluation form highlights many important laboratory skills. The pre-lab evaluation is at
bioeselfeval.html ; please fill out by Friday, January 23. The post-lab evaluation is at ; please fill out by Friday February 27.

Summary of Course Assignments
Item to be Graded Day of Week Due Date Time Location
Lab Notebook Pages Monday Jan. 26, Feb. 2, 9, 16, 23 1:00 p.m. Box in Keck 108 (lab)
Poster on HDF Cells - Draft Friday Feb. 20 2:00 p.m. Box in Keck 108 (lab) - critique at 2:00 p.m.
Poster on HDF Cells - Final Friday Feb. 27 4:30 p.m. BIOE Dept Office - Keck 116
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