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Overview: [Characterization
of red cell membrane proteins by SDS-PAGE] [research
paper]
Topics: [gel
analysis] [molecular
mass standard curve] [measuring
relative mobility] ["Hall
of Shame"]
Data: [gel
images]
Bands Crowded at the Top of the Gel
Stopped too soon – example 1
It is obvious where the dye front was on this gel.
There was room to continue electrophoresis, which
would have spread out the bands even more and improved
resolution. Note that the pattern is consistent with
the other gels that "went the distance." Relative mobility,
for gels of identical composition, is the same for
identical proteins. The resolution is improved with
the distance the proteins are permitted to migrate,
until resolution becomes limited by diffusion.
Stopped too soon – example 2
Each of these gels – one of about 7%T and one about 14%T – was
looking good. Someone became impatient and stopped the
run early. They are usable, but the separation would have been
much better if the dye front had been allowed to reach the bottom
of the gel. This will happen when you run late, the instructor
gets hungry, and you aren't there to keep him from taking down
your gel.
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