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Welcome again to BIOC 318. On the home page you will find a brief introduction to the course and a description of the overall course objectives and expected learning outcomes. In addition, please look over the following information about the course, instructors, and policies. Following the course policies is a complete timetable of scheduled activities and assignments with dates, times, and locations when applicable.

The information contained in this syllabus and related pages may be subject to change, with reasonable notice, at the discretion of the instructor.

Instructors and office hours

David Caprette, Teaching Professor
ABL 327 – third floor Anderson Biological laboratory Building
caprette@rice.edu, 713-348-3498 (campus extension 3498)
Office hours are variable. Please see the table at the bottom of this page.

Charles Stewart, Professor of Biochemistry
GBH W104
crs@rice.edu, 713-348-4926 (campus extension 4926)
Office hours TBD

Preparing for the course

Please plan to apply the skills and best practices that you learned in Bioc 211, Intermediate Experimental Biosciences (course prerequisite) and in Bioc 311, Advanced Experimental Biosciences, if you have had the opportunity to complete that course.  Please check the Course Schedule (below), review the meeting schedule for your lab section, and block out the scheduled times on your calendar. You must not schedule events that conflict with any of the listed in-lab activities, nor let anyone schedule a conflicting event for you.

Required materials

You will need eye protection for this course, consisting of a pair of lab goggles or laboratory safety glasses with side protection. A lab coat will be provided. There is no textbook but you will need to obtain a laboratory notebook. You are welcome to start with a partly filled notebook from another course, even one obtained from another student. New notebooks can be purchased from the campus store or ordered directly from the distributor. Please purchase a Hayden-McNeil Carbonless TOP Permanent Lab Notebook, 50 Set or 100 Set from Amazon, from WH Freeman, or the Campus store (ISBNs below).

Chemistry Top Bound 100 pages, ISBN 978-1-930882-00-3
or
Chemistry Top Bound 50 pages, ISBN 978-1-930882-50-8

Course timetable

All meetings begin at 1 pm except for the introductory meeting on Wednesday Feb 22, which will begin at 2 pm. All meetings will be held in ABL 219 with the exception of the classroom meetings Feb 22-23. Documents listed under Preparation can be found in the Resources section on Owl-Space and are also accessible from the side menu on the BIOC 318 web pages.

Sec 001 Sec 002 Preparation Description of activities

 

Weds Feb 22, 2:00 pm, Sewall Hall Rm. 307

 

Thur Feb 23, 1:00 pm, Sewall Hall Rm. 560

  • Review the course home page and syllabus and have questions ready
  • Review the documents "Aseptic technique," "Making serial dilutions," and Preparing spread plates," and be prepared for a quiz on the material
  • Introductory classroom meeting
  • Short quiz, lab tour, and practice sessions for dilutions and spreading plates

 

Mon Feb 27

 

Tues Feb 28

  • Review the documents "Isolating colonies" and "Colony morphology;" be prepared for a brief quiz
  • Review the PowerPoint slides in "Course introduction," posted in Resources and on the web
  • Peruse the document "culture media;" you will not be quizzed on this material
  • Prepare agar media and pour plates to be used for isolating colonies
  • Dilute and spread water samples
  • Practice sessions on isolating colonies and making streak plates

 

Weds Mar 1

 

Thur Mar 2

  • Review the documents "Agar slant tubes," "Gram stain," and "Relationship to oxygen," and be prepared for a short quiz
  • Prepare agar media for slant tubes and for plates to be used for anaerobic incubations next week
  • Identify and describe unique colonies on spread plates, streak out onto fresh agar plates
Mon Mar 6 Tues Mar 7
  • Review previous documents as needed
  • Consolidate isolates, conduct anaerobic incubations
  • Practice making Gram stains
  • (Tues section only) Gram stain isolates on aerobically incubated plates from the Monday group

Weds Mar 8

Thurs Mar 9
  • Again review the document "Relationship to oxygen"
  • Peruse the document "Using inoculating sticks;" you may prefer this technique over the technique that employs a flame and inoculating loop
  • Review the document "Using Bergey's manuals"
  • Evaluate anaerobically incubated plates for growth
  • Transfer isolates to agar slant tubes for temporary storage
  • Sign out a Bergey's manual
  • (Weds section only) Gram stain isolates on aerobically incubated plates from the Tuesday group
  • Assemble notebook copy pages, turn in for mid-course evaluation
SPRING BREAK
Mon Mar 20 Tues Mar 21
  • Review the document "Lab responsibilities" and think about which sets of responsibilities you would like to take on
  • Review the document "Viewing Gram stains;" there may be a short quiz
  • As you work with the microscope you may want to consult the document "Microscopy procedures," a step by step guide to using our Nikon Labophot microscopes
  • Streak out isolates onto fresh plates to rejuvenate cultures
  • Learn and practice microscopic examination of Gram stains
  • Evaluate Gram stains
  • Sign up for one set of lab responsibilities and arrange for training if necessary
Weds Mar 22 Thurs Mar 23
  • Enter observations for your isolates into the spreadsheet in Google Sheets
  • Review the document "Viewing living bacteria;" there may be a short quiz
  • Learn and practice microscopic observation of living bacteria
  • Record observations from microscopic examination of wet mounts of your isolates
Mon Mar 27 thru Weds Apr 19 Tues Mar 28 thru Thurs Apr 20
  • Assign isolates to Bergey's Groups, using recorded observations and in consultation with team members
  • Guided by Bergey's tables, plan strategies for identifying isolates
  • Peruse the following resources as needed: "Assays," "Assays illustrated," "Characterization writeup," and "Project proposal guidelines"
  • Conduct oxidase and catalase tests on all isolates
  • Conduct additional observations and assays as needed to identify a selected isolate to the species level
  • Write up your complete characterization of one isolate – upload into Drop Box on or before Friday April 14
  • Prepare a proposal for a strategy to isolate rare and/or "uncultivable" bacteria from our water source; up to 3 students may author this assignment, due on or before Friday April 21 in participant's Drop Box

Course grade

Your grade will be based upon your preparation and lab performance (20%), self reliance and contribution to team efforts (10%), laboratory notebook (20%), a monograph on a single bacterial species that you isolate and characterize (25%), and a team-written strategy for isolating rare & difficult to cultivate bacteria (25%).

Additional information and policies

Absences. Because the work in this course is part of a long term goal-oriented project, the concept of a "make-up lab" does not apply. Missing an important event is a lost opportunity that cannot be made up. As far as your course grade is concerned, absences from required meetings will be excused for the usual reasons, e.g., illness or family emergency. Absences due to circumstances within a student's control (e.g., forgetting to show up or travel for interviews) cannot be excused and will be reflected in the performance grade.

Lab safety. All participants must engage in safe laboratory practices and abide by specific "rules" set down for this course.

Late assignments. An assignment that is submitted late will lose 10% per day including holidays and weekends.

Honor code policy. As with all biosciences courses, the Rice University Honor Code applies to all personal conduct and assignments. All work submitted under your name must be your own work, not copied from another source nor completed with unauthorized assistance. Compliance with the honor code includes providing accurate information when requested, such as honest evaluations of yourself and your teammates.

Students with disabilities. Any student with a disability needing academic adjustments or accommodations should contact Dr. Caprette or Dr. Stewart as soon as possible and also contact Disabled Student Services in the Ley Student Center.

Instructor office hours

Week beginning

Monday Tuesday Wednesday Thursday Friday
Jan 9 11:00 am - 12:30 pm 12:30 - 2:00 pm office hrs by appt office hrs by app office hrs by appt
Jan 16 MLK HOLIDAY 12:30 - 2:00 pm 11:00 am - 12:30 pm 12:30 - 2:00 pm office hrs by appt
Jan 23 11:00 am - 12:30 pm 12:30 - 2:00 pm office hrs by appt 12:30 - 2:00 pm office hrs by appt
Jan 30 11:00 am - 12:30 pm 12:30 - 2:00 pm 9:30 am - 11:00 pm 12:30 - 2:00 pm office hrs by appt
Feb 6 11:00 am - 12:30 pm 12:30 - 2:00 pm office hrs by appt BREAK BREAK
Feb 13 11:00 am - 12:30 pm 12:30 - 2:00 pm office hrs by appt 12:30 - 2:00 pm office hrs by appt
Feb 20 11:00 am - 12:30 pm 12:30 - 2:00 pm office hrs by appt office hrs by appt office hrs by appt
Feb 27 11:00 am - 12:30 pm office hrs by appt office hrs by appt office hrs by appt office hrs by appt
Mar 6 office hrs by appt office hrs by appt 11:00 am - 12:30 pm office hrs by appt office hrs by appt
Mar 13 SPRING BREAK SPRING BREAK SPRING BREAK SPRING BREAK SPRING BREAK
Mar 20 office hrs by appt office hrs by appt office hrs by appt office hrs by appt office hrs by appt
Mar 27 11:00 am - 12:30 pm office hrs by appt office hrs by appt office hrs by appt office hrs by appt
Apr 3 11:00 am - 12:30 pm office hrs by appt office hrs by appt office hrs by appt office hrs by appts
Apr 10 11:00 am - 12:30 pm office hrs by appt 11:00 am - 12:30 pm office hrs by appt office hrs by appt
Apr 17 11:00 am - 12:30 pm office hrs by appt office hrs by appt office hrs by appt office hrs by appt

Created by David R. Caprette, Teaching Professor, Rice University (caprette@rice.edu) 21 Dec 16] for the course BIOC 318, Laboratory Studies in Microbiology. Please feel free to copy and/or modify these materials for use in your own academic or other nonprofit program. If you don't mind letting me know of such intentions I'll be happy to hear from you.
Last updated 21 Feb 2017