BIOE442 - TISSUE ENGINEERING LABORATORY

Lab Protocol

Table of Contents

GPC Sample Preparation
GPC Operation
GPC FOR PLLA AND PLGA
PLLA/PLGA Polymer Film Fabrication
Degradation Study of PLLA and PLGA
Synthesis of Poly-(L-Lactic Acid)
Synthesis Setup (PPT)
FTIR
Differential Scanning Calorimetry (DSC)
Cell Studies on PLLA Films

Attachments
• Polymer Introduction - Background Information on GPC
• Information on Gloves: Dichloromethane, Hexane, Chloroform, Methanol, and Acetone

Handouts
•  I. Engelberg and J. Kohn. “Physico-mechanical properties of degradable polymers used in medical applications: a comparative study.” Biomaterials , 12 : 292-304, 1991.
•  L. Lu, C. Garcia, and A. Mikos. “ In vitro degradation of thin poly(DL-lactic- co -glycolic acid) films.” Journal of Biomedical Materials Research , 46 : 236-244, 1999.
•  S. Ishaug, M. Yaszemski, R. Bizios, A. Mikos. “Osteoblast function on synthetic biodegradable polymers.” Journal of Biomedical Materials Research , 28 : 1445-1453, 1994.

Assistance in protocol development from Heungsoo Shin, John Fisher and Mark Timmer is acknowledged.

ã Copyright, Dr. Ann Saterbak, August 2001, August 2002.