BIOE 441: TISSUE ENGINEERING MODULE

 

Tissue Engineering Module Schedule............................................................................... 1

GPC Sample Preparation................................................................................................ 12

GPC Operation................................................................................................................ 14

GPC Data Analysis......................................................................................................... 16

GPC for PLLA and PLGA............................................................................................... 17

PLLA/PLGA Polymer Film Fabrication.......................................................................... 18

Degradation Study of PLLA and PLGA........................................................................... 20

Synthesis of Poly-(L-Lactic Acid), Part I-Chemical Synthesis........................................ 24

Synthesis of Poly-(L-Lactic Acid), Part II-Polymer Clean-up......................................... 31

FTIR        ....................................................................................................................... 34

DSC         ....................................................................................................................... 36

Cell Studies on PLLA Films........................................................................................... 38

HDF Viability on PLLA Films........................................................................................ 41

HDF Attachment on PLLA Films.................................................................................... 42

HDF Proliferation on PLLA Films.................................................................................. 44

 

Attachments

·       Polymer Introduction - Background Information on GPC

·       Information on Gloves:  Dichloromethane, Hexane, Chloroform, Methanol, and Acetone

 

Handouts

·       I. Engelberg and J. Kohn.  “Physico-mechanical properties of degradable polymers used in medical applications:  a comparative study.” Biomaterials, 12: 292-304, 1991.

·       L. Lu, C. Garcia, and A. Mikos.  “In vitro degradation of thin poly(DL-lactic-co-glycolic acid) films.”   Journal of Biomedical Materials Research, 46: 236-244, 1999.

·       S. Ishaug, M. Yaszemski, R. Bizios, A. Mikos.  “Osteoblast function on synthetic biodegradable polymers.” Journal of Biomedical Materials Research, 28: 1445-1453, 1994.

 

 

Assistance in protocol development from Heungsoo Shin, John Fisher and Mark Timmer is acknowledged.

 

ã Copyright, Dr. Ann Saterbak, August 2001, August 2002.