Arabidopsis genes apparently encoding triterpenoid
biosynthetic enzymes
FPP synthases
At number |
Gene name |
T-DNA |
Mutant name |
Location |
Status |
At5g47770 |
FPS1 |
SALK_004298 |
fps1-1 |
exon 2 S |
homozygous |
|
|
SALK_037778 |
fps1-2 |
58 bp 5’ S |
homozygous |
|
|
SALK_051136 |
fps1-3 |
78 bp 5’ S |
homozygous |
|
|
SALK_056888 |
fps1-4 |
59 bp 5’ S |
homozygous |
|
|
SALK_073576 |
fps1-5 |
exon 2 S |
homozygous |
|
|
SALK_106128 |
fps1-6 |
|
no insert |
|
|
SALK_106120 |
fps1-7 |
253 bp 5' S |
low priority |
At4g17190 |
FPS2 |
SALK_045864 |
fps2-1 |
195 bp 5’ S |
homozygous |
|
|
GABI_360G12 |
fps2-2 |
exon 9 S |
homozygous |
Squalene synthases
At number |
Gene name |
T-DNA |
Mutant name |
Location |
Status |
At4g34640 |
SQS1 |
SALK_077057 |
sqs1-1 |
181 bp 5’ S |
homozygous |
|
|
SALK_034266 |
sqs1-2 |
|
no insert? |
|
|
SALK_034431 |
sqs1-3 |
|
no insert? |
|
|
SALK_087515 |
sqs1-4 |
exon 6 S |
homoz. lethal |
At4g34650 |
SQS2 |
SALK_018698 |
sqs2-1 |
|
no insert |
|
|
SALK_018690 |
sqs2-2 |
212 bp 3' S |
homozygous |
|
|
GABI_651C05 |
sqs2-3 |
exon 9 |
in progress |
Squalene epoxidases
At number |
Gene name |
T-DNA |
Mutant name |
Location |
Status |
At1g58440 |
|
SALK_053978 |
|
|
no insert |
|
|
SALK_022763 |
|
exon 7 S |
small, sterile |
|
|
SALK_016111 |
|
152 bp 5’ S |
homozygous |
|
|
SALK_133684 |
|
|
no insert |
|
|
SALK_138454 |
|
300 bp 5’ S |
homozygous |
|
|
CSHL_ET3215 |
|
intron 5 |
small, sterile |
At2g22830 |
|
SALK_016514 |
|
intron 1 S |
homozygous |
|
|
SALK_064182 |
|
exon 3 S |
homozygous |
At4g37760 |
|
SALK 094174 |
|
481 bp 5’ S |
homozygous |
|
|
SAIL_723_F01 |
|
exon 2 |
in progress |
At5g24140 |
|
SALK_012382 |
|
|
no insert |
|
|
SALK_040805 |
|
exon 7 S |
homozygous |
|
|
SALK_012094 |
|
exon 7 S |
homozygous |
At5g24150 |
|
SALK_082809 |
|
|
no insert |
|
|
SALK_024504 |
|
429 bp 5’ S |
homozygous |
|
|
GT_5_7074 |
|
exon 4 |
in progress |
At5g24160 |
|
SALK_112366 |
|
|
no insert |
|
|
SALK_083343 |
|
intron 1 S |
homozygous |
|
|
SALK_118625 |
|
exon 7 S |
homozygous |
|
|
WiscDsLox365A10 |
|
54-103 bp 5’ |
in progress |
|
|
WiscDsLox233D7 |
|
exon 1-5’ |
in progress |
Oxidosqualene cyclases
At number |
Gene name |
T-DNA or TILLING (CS) |
Mutant name |
Location |
Status |
At2g07050 |
CAS1 |
SALK_056272 |
cas1-2 |
intron 15 S |
homoz. lethal |
|
|
SALK_119879 |
cas1-3 |
exon 7 S |
homoz. lethal |
|
|
CS93266
(TILLING) |
cas1-4 |
splice junction (G280A) S |
homoz. lethal |
|
|
CS85907
(TILLING) |
cas1-5 |
E561K (conserved residue) S |
homoz. lethal |
|
|
CS92277
(TILLING) |
cas1-6 |
D586N (unconserved residue) S |
homozygous |
|
|
CS90185
(TILLING) |
cas1-7 |
D603N (conserved residue) S |
homoz. lethal |
At1g78970 |
LUP1 |
SALK_032555 |
lup1-3 |
intron 11 S |
homozygous |
|
|
SALK_035461 |
lup1-4 |
intron 13 |
homozygous |
|
|
SALK_122328 |
lup1-5 |
intron 15 S |
low priority |
|
|
SALK_088765 |
lup1-6 |
|
no insert |
|
|
SALK_088764 |
lup1-7 |
intron 13 S |
low priority |
|
|
SALK_097669 |
lup1-8 |
|
no insert |
|
|
WiscDsLox376B02 |
lup1-9 |
intron 11 |
ordered |
At1g78960 |
LUP2 |
SALK_013690 |
lup2-1 |
exon 15 |
homozygous |
|
|
SALK_103917 |
lup2-2 |
intron 2 S |
in progress |
|
|
SALK_104920 |
lup2-3 |
130 bp 3' S |
in progress |
|
|
SALK_024920 |
lup2-4 |
intron 10 S |
in progress |
At1g78950b |
LUP3 |
SALK_000127 |
lup3-1 |
intron 1 S |
in progress |
|
|
SALK_028737 |
lup3-2 |
|
no insert? |
|
|
SALK_137624 |
lup3-3 |
exon 7 S |
in progress |
|
|
WiscDslox390G02 |
lup3-4 |
~200 bp 5' |
ordered |
At1g78950a |
LUP4 |
SALK_048237 |
lup4-1 |
exon 15 |
homozygous |
|
|
SALK_073455 |
lup4-2 |
intron 4 S |
in progress |
|
|
SALK_026433 |
lup4-3 |
|
no insert |
At1g66960 |
LUP5 |
SALK_073124 |
lup5-1 |
intron 12 S |
low priority |
|
|
SALK_022044 |
lup5-2 |
exon 9 S |
homozygous |
|
|
SALK_094474 |
lup5-3 |
intron 9 S |
homozygous |
At4g15340 |
PEN1 |
SALK_018285 |
pen1-1 |
exon 1 S |
homozygous |
|
|
SALK_067736 |
pen1-2 |
intron 5 S |
homozygous |
|
|
SALK_134700 |
pen1-3 |
intron 5 |
low priority |
|
|
SALK_052733 |
pen1-4 |
intron 10 |
low priority |
At4g15370 |
PEN2 |
SALK_069612 |
pen2-1 |
|
no insert |
|
|
SALK_071455 |
pen2-2 |
exon 2 |
in progress |
|
|
GABI_039B06 |
pen2-3 |
exon 2 S |
in progress |
|
|
WiscDsLox356G07 |
pen2-4 |
intron 1 |
ordered |
At5g36150 |
PEN3 |
SALK_029567 |
pen3-1 |
exon 4 S |
homozygous |
|
|
SALK_047549 |
pen3-2 |
219 bp 5’ |
homozygous |
|
|
SALK_116161 |
pen3-3 |
intron 6 |
in progress |
At5g48010 |
PEN4 |
SALK_064244 |
pen4-1 |
intron 8 S |
homozygous |
|
|
SALK_111741 |
pen4-2 |
exon11 |
low priority |
|
|
SALK_111739 |
pen4-3 |
|
no insert |
|
|
SALK_138058 |
pen4-4 |
exon 13 S |
homozygous |
|
|
SALK_111730 |
pen4-5 |
|
no insert |
At5g42600 |
PEN5 |
GABI_344D01 |
pen5-1 |
|
no insert |
|
|
SALK 152492 |
pen5-2 |
intron 3 |
in progress |
At1g78500 |
PEN6 |
SALK_060682 |
pen6-1 |
exon 9 S |
homozygous |
|
|
SALK_036693 |
pen6-2 |
exon 14 |
ordered |
At3g45130 |
PEN7 |
SALK_003062 |
pen7-1 |
|
no insert |
|
|
SALK_128191 |
pen7-2 |
intron 9 S |
homozygous |
CPI1
At number |
Gene name |
T-DNA or TILLING (CS) |
Mutant name |
Location |
Status |
At5g50375 |
CPI1 |
SALK_053963 |
cpi1-1 |
119 bp 5’ |
no insert? |
|
|
SALK_135330 |
cpi1-2 |
100 bp 5’ |
in progress |
Location of the insert as reported by the generating facility. We are confirming these locations by sequencing the relevant PCR products. S Indicates that the insert location has been confirmed by sequencing.
Homozygous: progeny of plants scored as homozygous by PCR. Homozygous lethal: lines for which a verified heterozgote has not produced any homozygous mutants after at least two generations in our lab. In progress: lines obtained from the ABRC stock center and verified by PCR to have the T-DNA insert of interest. No insert: PCR analysis failed to detect reported insert in line. Low priority: "in progress" lines not currently pursued because at least two homozygous lines with verified inserts in the same gene have been obtained. Ordered: lines ordered or obtained but not yet verified to have the T-DNA of interest.
updated 3-12-05, BB