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Arabidopsis triterpenoids

Using functional genomics to determine how and why plants synthesize diverse triterpenoids
(back to triterpenoids)


A project at Rice University
supported by the National Science Foundation

Arabidopsis genes apparently encoding triterpenoid biosynthetic enzymes


FPP synthases
At number
Gene name
T-DNA
Mutant name
Location
Status

At5g47770

FPS1

SALK_004298

fps1-1

exon 2 S

homozygous

SALK_037778

fps1-2

58 bp 5’ S

homozygous

SALK_051136

fps1-3

78 bp 5’ S

homozygous

SALK_056888

fps1-4

59 bp 5’ S

homozygous

SALK_073576

fps1-5

exon 2 S

homozygous

SALK_106128

fps1-6

no insert

SALK_106120

fps1-7

253 bp 5' S

low priority

At4g17190

FPS2

SALK_045864

fps2-1

195 bp 5’ S

homozygous

GABI_360G12

fps2-2

exon 9 S

homozygous


Squalene synthases

At number
Gene name
T-DNA
Mutant name
Location
Status

At4g34640

SQS1

SALK_077057

sqs1-1

181 bp 5’ S

homozygous

SALK_034266

sqs1-2

no insert?

SALK_034431

sqs1-3

no insert?

SALK_087515

sqs1-4

exon 6 S

homoz. lethal

At4g34650

SQS2

SALK_018698

sqs2-1

no insert

SALK_018690

sqs2-2

212 bp 3' S

homozygous

GABI_651C05

sqs2-3

exon 9

in progress


Squalene epoxidases

At number
Gene name
T-DNA
Mutant name
Location
Status

At1g58440

SALK_053978

no insert

SALK_022763

exon 7 S

small, sterile

SALK_016111

152 bp 5’ S

homozygous

SALK_133684

no insert

SALK_138454

300 bp 5’ S

homozygous

CSHL_ET3215

intron 5

small, sterile

At2g22830

SALK_016514

intron 1 S

homozygous

SALK_064182

exon 3 S

homozygous

At4g37760

SALK 094174

481 bp 5’ S

homozygous

SAIL_723_F01

exon 2

in progress

At5g24140

SALK_012382

no insert

SALK_040805

exon 7 S

homozygous

SALK_012094

exon 7 S

homozygous

At5g24150

SALK_082809

no insert

SALK_024504

429 bp 5’ S

homozygous

GT_5_7074

exon 4

in progress

At5g24160

SALK_112366

no insert

SALK_083343

intron 1 S

homozygous

SALK_118625

exon 7 S

homozygous

WiscDsLox365A10

54-103 bp 5’

in progress

WiscDsLox233D7

exon 1-5’

in progress


Oxidosqualene cyclases

At number
Gene name
T-DNA or TILLING (CS)
Mutant name
Location
Status

At2g07050

CAS1

SALK_056272

cas1-2

intron 15 S

homoz. lethal

SALK_119879

cas1-3

exon 7 S

homoz. lethal

CS93266
(TILLING)

cas1-4

splice junction (G280A) S

homoz. lethal

CS85907
(TILLING)

cas1-5

E561K (conserved residue) S

homoz. lethal

CS92277
(TILLING)

cas1-6

D586N (unconserved residue) S

homozygous

CS90185
(TILLING)

cas1-7

D603N (conserved residue) S

homoz. lethal

At1g78970

LUP1

SALK_032555

lup1-3

intron 11 S

homozygous

SALK_035461

lup1-4

intron 13

homozygous

SALK_122328

lup1-5

intron 15 S

low priority

SALK_088765

lup1-6

no insert

SALK_088764

lup1-7

intron 13 S

low priority

SALK_097669

lup1-8

no insert

WiscDsLox376B02

lup1-9

intron 11

ordered

At1g78960

LUP2

SALK_013690

lup2-1

exon 15

homozygous

SALK_103917

lup2-2

intron 2 S

in progress

SALK_104920

lup2-3

130 bp 3' S

in progress

SALK_024920

lup2-4

intron 10 S

in progress

At1g78950b

LUP3

SALK_000127

lup3-1

intron 1 S

in progress

SALK_028737

lup3-2

no insert?

SALK_137624

lup3-3

exon 7 S

in progress

WiscDslox390G02

lup3-4

~200 bp 5'

ordered

At1g78950a

LUP4

SALK_048237

lup4-1

exon 15

homozygous

SALK_073455

lup4-2

intron 4 S

in progress

SALK_026433

lup4-3

no insert

At1g66960

LUP5

SALK_073124

lup5-1

intron 12 S

low priority

SALK_022044

lup5-2

exon 9 S

homozygous

SALK_094474

lup5-3

intron 9 S

homozygous

At4g15340

PEN1

SALK_018285

pen1-1

exon 1 S

homozygous

SALK_067736

pen1-2

intron 5 S

homozygous

SALK_134700

pen1-3

intron 5

low priority

SALK_052733

pen1-4

intron 10

low priority

At4g15370

PEN2

SALK_069612

pen2-1

no insert

SALK_071455

pen2-2

exon 2

in progress

GABI_039B06

pen2-3

exon 2 S

in progress

WiscDsLox356G07

pen2-4

intron 1

ordered

At5g36150

PEN3

SALK_029567

pen3-1

exon 4 S

homozygous

SALK_047549

pen3-2

219 bp 5’

homozygous

SALK_116161

pen3-3

intron 6

in progress

At5g48010

PEN4

SALK_064244

pen4-1

intron 8 S

homozygous

SALK_111741

pen4-2

exon11

low priority

SALK_111739

pen4-3

no insert

SALK_138058

pen4-4

exon 13 S

homozygous

SALK_111730

pen4-5

no insert

At5g42600

PEN5

GABI_344D01

pen5-1

no insert

SALK 152492

pen5-2

intron 3

in progress

At1g78500

PEN6

SALK_060682

pen6-1

exon 9 S

homozygous

SALK_036693

pen6-2

exon 14

ordered

At3g45130

PEN7

SALK_003062

pen7-1

no insert

SALK_128191

pen7-2

intron 9 S

homozygous


CPI1

At number
Gene name
T-DNA or TILLING (CS)
Mutant name
Location
Status

At5g50375

CPI1

SALK_053963

cpi1-1

119 bp 5’

no insert?

SALK_135330

cpi1-2

100 bp 5’

in progress


Location of the insert as reported by the generating facility.  We are confirming these locations by sequencing the relevant PCR products.  S Indicates that the insert location has been confirmed by sequencing.

Homozygous: progeny of plants scored as homozygous by PCR.  Homozygous lethal: lines for which a verified heterozgote has not produced any homozygous mutants after at least two generations in our lab.  In progress: lines obtained from the ABRC stock center and verified by PCR to have the T-DNA insert of interest.  No insert:  PCR analysis failed to detect reported insert in line.  Low priority: "in progress" lines not currently pursued because at least two homozygous lines with verified inserts in the same gene have been obtained.  Ordered: lines ordered or obtained but not yet verified to have the T-DNA of interest.

updated 3-12-05, BB


Biochemistry