Development of an array imaging method for DNA multiple SNP
determinations for diagnosticsand genotyping and ultimately for sequencing Exploratory research aimed at
developing a method for simultaneously determining many genetic anomalies,
primarily SNPs, without amplification of the genetic material through
whole-chip imaging. The chip is to be covered with thousands of primer features
with each feature containing~100000 primers complementary to the region just
before a SNP location. Terminating bases labeled with four different dyes and
polymerase add a single labeled base to the primer. Four lasers of different
wavelengths matching the dyes read the whole chip’s fluorescence. Infrared
laser trace gas monitoring instrumentation development (in collaboration
with Frank
K. Tittel)