Recordkeeping, Writing,
& Data Analysis


Microscope studies

Flagella experiment
Laboratory math
Blood fractionation
Gel electrophoresis
Protein gel analysis
Concepts/ theory
Keeping a lab notebook
Writing research papers
Dimensions & units
Using figures (graphs)
Examples of graphs
Experimental error
Representing error
Applying statistics
Principles of microscopy

Solutions & dilutions
Protein assays
Fractionation & centrifugation
Radioisotopes and detection

Selected Laboratory Methods

This section deals with instrumentation and methodology that is applicable to a variety of laboratory studies. The information here might serve as a suitable reference for future laboratory work.


Presented are principles of bright field, oil immersion, dark field, phase contrast, and D.I.C. optics and recommended specimens on which to practice. Methods for measurement with a light microscope are described, including use of a cell counting chamber (hemacytometer) and eyepiece reticule.

Solutions & dilutions

This section distinguishes among types of mixtures and defines the term solution. It describes units and prefixes for volume, amount, and concentration, and presents types of formulas that are used to describe solutions. Examples of how to make solutions are presented. This section also describes how to dilute a solution to final desired concentration or to final concentration and volume, again with examples.

Protein assays

A general approach to setting up and interpreting a colorimetric assay is presented here. The principles are not limited to protein assays, however the remainder of this section describes assays for protein that have been employed in the past, some of which are still in common use.

Spectrophotometry & Beer's Law

This section is brief (one page only), but it is important. It includes a procedure for calibrating a relatively simple spectrophotometer, the Spectronic 20.


Here you will find a general scheme for "taking apart" cells and tissues, and separating components by a process called differential centrifugation. It includes the concepts of fractions and yields, describes some limitations on reporting yields, and describes how information related to fractionation is typically presented in a paper.

Copyright and Intended Use
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Created by David R. Caprette (caprette@rice.edu), Rice University 10 Aug 12