Welcome to Bioc 415

• Syllabus
• Assignments

Scheduled labs

• First session
• Membrane labs
• Nerve labs
• Nerve/muscle labs

On-line resources

Introductory lab manual (Bioc 211)
Bioc 311 resources

Bioc 415 manual (pdf)

Bioc 415 appendixes (pdf)

Raw Data for Membrane Potentials

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[data from 1998]
[data from 2000]

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The data below were generated spring 2000.  



Data from three experimental designs are reported.			
All transmembrane potentials are reported in |mV|.			
Report data as mean ± standard deviation.			
When data are plotted, use mean ± standard error of the mean.			
			
Question:  Is electrogenic activity of the pump responsible for			
     the entire membrane potential?			
			
Contributors:  	Stephanie Tharpe, Theresa Trevino, Jonathan Lee		
			
	Control	After washout	
Control	Ringers with	with control	
Ringers	6 mM oubain	Ringers	
74.9	58.5	62.3	
71.5	62.2	66.0	
75.2	60.1	70.6	
78.6	51.8	60.8	
76.0	51.8	65.8	
65.7	72.7	54.9	
69.6	69.8	66.3	
76.1	64.3	69.8	
71.8	61.6	60.6	
73.1	61.6	69.7	
			
			
Questions:  Can chemical concentration gradients, coupled with a semipermeable membrane,			
   account for the membrane potential?  Will the Em respond qualitatively or quantitatively			
   to changes in the extracellular concentration of potassium ion?			
			
Contributors:  	Betty Nguyen, Saheel Sutaria, Brian Green		
			
			
			
Control	Ringers with	Ringers with	Ringers with
Ringers	1.6 mM Kout	10.7 mM Kout	26.8 mM Kout
65	77	60	46
58	71	49	53
41	66	59	35
56	74	47	40
59	66	43	32
56	68	58	39
51	64	64	42
56	67	54	44
56	63	45	
55	60	44	

Compare with Nernst values to test equilibrium potential model.			
Compare (on same plot) with Goldman values to test diffusion potential model			
		
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