Welcome to Bioc 415

Course activities

On-line resources

Introductory lab manual (Bioc 211)
Bioc 311 resources

Bioc 415 manual (pdf)

Bioc 415 appendixes (pdf)

Experimental Physiology: Introduction

The opening meeting is essential. It and all other meetings will start on time. If a situation that is beyond your control is likely to prevent you from making it to class on time, then please inform the instructor.

Introductory discussion (Monday 22 Aug – 1 pm ABL 329)

  • Course introduction and survey
  • Laboratory projects and assignments
  • Kinds of data to be collected
  • Some types of transducers used for physiology
  • Measuring membrane potentials
  • Calibrating and using an intracellular (IC) probe
  • Analog-to-digital (A/D) data acquisition

Prior to the first laboratory meeting

  • Review principles for recording data (Appendix A, manual)
  • Read Appendix B, Analog-to-digital (A/D) data acquisition
  • Review the basic principles of electricity, including DC electricity and Ohm's law and the concept of capacitance
  • Be prepared to use Ohm's Law to determine electrical resistance, voltage, or current.
  • Be prepared for a possible quiz on the reading matter and/or subject matter covered today; quizzes will be unannounced

Laboratory (Wednesday 24 Aug – 2:15 pm GRB W100)

  • Self-paced tutorial using data acquisition and analysis software
  • Explore recording of slow and fast signals using filters
  • Calibrate an IC probe

Preparation for next time

  • Read chapter 1 of the laboratory manual on the origin of the membrane potential, up to page 11 on the diffusion potential model
  • Read Appendix C of the manual on designing experiments
  • [ASSIGNMENT] Prepare an experimental design for testing the contribution of the electrogenic property of sodium pump to the membrane potential, to be brought to the next class
  • Be prepared for a possible quiz on the reading or subject matter covered today

Created by David R. Caprette (caprette@rice.edu), Rice University 30 Mar 00
Updated 14 Aug 11
Copyright and Intended Use