Table of Contents

 

BIOE 342 Section 001 Schedule....................................................................................... 1

BIOE 342 Section 002 Schedule....................................................................................... 3

BIOE 342 Section 003 Schedule....................................................................................... 5

BIOE 342 Section 004 Schedule....................................................................................... 7

Micropipette Exercise....................................................................................................... 9

Visualization of Cells...................................................................................................... 11

Contamination Exercise.................................................................................................. 13

pH and Color of Media................................................................................................... 15

Practice Sterile Technique.............................................................................................. 16

Tips for Sterile Technique and Cell Manipulation.......................................................... 18

Feeding Cells.................................................................................................................. 20

Cell Passage.................................................................................................................... 21

Determination of Cell Concentration............................................................................... 24

Hemocytometer Instructions............................................................................................ 26

Use of Coulter Counter.................................................................................................... 27

Z1 Routine Cell Counting................................................................................................ 29

Z2 Routine Cell Counting................................................................................................ 30

Z2 Cell Channelyzer........................................................................................................ 31

Fibronectin Attachment Assay......................................................................................... 32

MTT Viability Test......................................................................................................... 35

Instructions for Using the Genesys 10UV and 20 Spectrophometers............................... 39

Live/Dead Fluorescence Assay....................................................................................... 40

Nikon Fluorescence Microscope.................................................................................... 42

Zeiss Fluorescence Microscope...................................................................................... 43

Quantitative Cell Attachment Assay................................................................................ 44

Anti-PCNA Staining........................................................................................................ 47

Cell Proliferation Assay................................................................................................. 50

Transfection of GFP into CHO Cells.............................................................................. 54

 

Assistance in protocol development from Rachael Schmedlen and Andi Gobin is acknowledged.