Index
Z1 Routine Cell Counting
|
Screen |
Should Read |
To Change Settings |
Your Action |
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|
|
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To get to S1, hit Set-up. |
|
S1 |
100 mm C, Kd = 57.XX1 Select units: <mm> Set size: Y · Y should be 9.1-10 for CHO cells, 9.5-10.5 for HDF cells |
To change Y, down arrow to appropriate line and change number with numeric keypad. |
When size is set, hit Set-up. |
|
S2 |
Select aperture: <100 mm C> Aperture Kd: 57. XX1 Metered volume: <0.5 mL> Measure aperture: <No> Optimize settings: <Yes> |
Change Optimize settings to <Yes> by down arrow to last line, then right arrow to toggle. |
Once settings are correct, hit Start/Stop one or two times, as needed, until cell count begins. |
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1. Cell Conc should read between 0 and 5% 2. Bars on graph should be between one-third and one-half of height of graph. 3. Watch aperture for plugs of debris. 4. Listen for constant “ticks”. |
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2. If bars are not appropriate height, see notes below. 3./4. Hit Unblock if debris is blocking aperture. |
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A4 |
Count XXXX |
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1. Record this count. 2. To repeat measurement, hit Start/Stop. 3. To count another sample, rinse aperture. Place new CC vial on stand. Restart with screen S1. |
1 values of XX will vary depending on which Coulter Counter is being used. Do not adjust the Kd value.
If bars on graph are consistently above one-half of the height of the graph, increase the value of Y and/or X.
If bars on graph are consistently below one-third of the height of the graph, decrease the value of Y and/or X.
Z2 Routine Cell Counting
|
Screen |
Should Read |
To Change Settings |
Your Action |
|
|
|
|
To get to S1, hit Set-up. |
|
S1 |
100 mm C, Kd = 57. XX1 Select units: <mm> Set upper size TU: X · X should be 23 for CHO cells, 23 for HDF cells Set lower size TL: Y · Y should be 9.1-10 for CHO cells, 9.5-10.5 for HDF cells Count mode: <above TL > |
To change X or Y, down arrow to appropriate line and change number with numeric keypad. |
When size is set, hit Set-up. |
|
S2 |
Select aperture: <100 mm C> Aperture Kd: 57. XX1 Metered volume: <0.5 mL> Measure aperture: <No> Optimize settings: <Yes> |
Change Optimize settings to <Yes> by down arrow to last line, then right arrow to toggle. |
Once settings are correct, hit Start/Stop one or two times, as needed, until cell count begins. |
|
|
1. Cell Conc should read between 0 and 5%. 2. Bars on graph should be between one-third and one-half of height of graph. 3. Watch aperture for plugs of debris. 4. Listen for constant “ticks”. |
|
2. If bars are not appropriate height, see notes below. 3./4. Hit Unblock if debris is blocking aperture. |
|
A4 |
Count XXXX |
|
1. Record this count. 2. To repeat measurement, hit Start/Stop. 3. To count another sample, rinse aperture. Place new CC vial on stand. Restart with screen S1. |
1 values of XX will vary depending on which Coulter Counter is being used. Do not adjust the Kd value.
If bars on graph are consistently above one-half of the height of the graph, increase the value of Y and/or X.
If bars on graph are consistently below one-third of the height of the graph, decrease the value of Y and/or X.
Z2 Cell Channelyzer
If you want to get information on the particle size distribution of your cell sample, follow below:
|
Screen |
Should Read |
To change settings |
Your Action |
|
A4 |
Count XXXX |
|
Record this count. Hit Output. |
|
A7 |
Graph------- Mode <Stat> C1 <Q> C2 <R> Stat <Mean> |
Q & R are locations of cursor; use arrows to move cursors on graph. Within stats, can look at mean, mode, median, stdev, count, conc.; usually leave on Mean. |
If you want a print-out, hit Output until arrive at A10 screen. |
|
A10 |
Mode <Manual> Graph <Yes> Table <n/a> |
Change Form to <Long> by arrow down and arrow right to toggle. Change Graph to <Yes> by arrow down and arrow right to toggle. Change Table to <Diff Count> by arrow down and arrow right to toggle. |
Hit Output until arrive at A7 screen. Hit Print. |
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Hit Set-up to get back to S1 screen. |