Rice: Unconventional Wisdom
Placeholder Image

Metabolic Engineering and Synthetic & Systems Biology Laboratory

James Clomburg


James Clomburg

Education

2012 - Rice University, PhD in Chemical and Biomolecular Engineering
2006 - University of Texas, BS in Chemical Engineering

 

Professional Experience

2014 to Present - Research Scientist, Rice University, Chemical and Biomolecular Engineering
2012 to 2014 - Post-Doctoral Research Associate, Rice University, Chemical and Biomolecular Engineering

Projects

Jan 2013 to Present - Efficient synthesis of hydrocarbons using an engineered reversal of the β-oxidation cycle: A new paradigm for the production of advanced biofuels. J.M. Clomburg (Principal Investigator) and R. Gonzalez (Co-PI), Source of Support: National Science Foundation.
Jan 2013 to Present - A native pathway for the production of n-butanol in Escherichia coli: A new paradigm for synthetic biology. J.M. Clomburg (Principal Investigator) and R. Gonzalez (Co-PI), Source of Support: National Science Foundation.
Dec 2012 to Apr 2014 - Synthesis of ω-functionalized n-alcohols and carboxylic acids. R. Gonzalez and J.M. Clomburg (Co-Principal Investigators), Source of Support: BASF Corporation.

Publications

Full Publication List and Citation Details.

Kim, S., Clomburg, J.M., Gonzalez, R. (2015). Synthesis of medium-chain length (C6-C10) fuels and chemicals via β-oxidation reversal in Escherichia coli. Journal of Industrial Microbiology and Biotechnology. doi:10.1007/s10295-015-1589-6.

Clomburg, J.M., Blankschien, M.D., Vick, J.E., Chou, A., Kim, S., Gonzalez, R. (2015). Integrated engineering of β-oxidation reversal and ω-oxidation pathways for the synthesis of medium chain ω-functionalized carboxylic acids. Metabolic Engineering. doi:10.1016/j.ymben.2015.01.007.

Vick, J.E., Clomburg, J.M., Blankschien, M.D., Chou, A., Kim, S., Gonzalez, R. (2015). Escherichia coli enoyl-acyl carrier protein reductase (FabI) supports efficient operation of a functional reversal of the β-oxidation cycle. Applied and Environmental Microbiology. 81(1). doi:10.1128/AEM.03521-14.

Zhao, Z., Arentz, J., Pretzer, L. A., Limpornpipat, P., Clomburg, J. M., Gonzalez, R., Schweitzer, N. M., Wu, T., Miller, J. T., Wong, M. S. (2014). Volcano-shape glycerol oxidation activity of palladium-decorated gold nanoparticles. Chemical Science. 5(10), 3715. doi:10.1039/C4SC01001A.

Cintolesi, A., Clomburg, J.M., Gonzalez, R. (2014). In silico assessment of the metabolic capabilities of an engineered functional reversal of the β-oxidation cycle for the synthesis of longer-chain (C ≥ 4) products. Metabolic Engineering. DOI: 10.1016/j.ymben.2014.02.011.

Mattam A.J, Clomburg J.M., Gonzalez R., Yazdani S.S. (2013). Fermentation of glycerol and production of valuable chemical and biofuel molecules. Biotechnology Letters. 35(6): 831-842.

Clomburg, J.M., and Gonzalez, R. (2013). Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicalsTrends in Biotechnology. 31(1): 20-28.

Mazumdar, S., Blankschien, M. D., Clomburg, J. M., and Gonzalez, R. (2013). Efficient synthesis of L-lactic acid from glycerol by metabolically engineered Escherichia coliMicrobial Cell Factories. 12(7).

Clomburg, J.M., Vick, J.E., Blankschien, M. D., Rodriguez-Moya, M., Gonzalez, R. (2012). A synthetic biology approach to engineer a functional reversal of the beta-oxidation cycleACS Synthetic Biology. 1(11): 541-554.

Cintolesi, A.*, Clomburg, J.M.*, Rigou, V., Zygourakis, K., and Gonzalez, R. (2012). Quantitative analysis of the fermentative metabolism of glycerol in Escherichia coliBiotechnology and Bioengineering, 109 (1): 187-198. *Equal Contributions.

Clomburg, J.M., and Gonzalez, R. (2011) Metabolic Engineering of Escherichia coli for the Production of 1,2-Propanediol from Glycerol. Biotechnology and Bioengineering, 108 (4): 867-879..

Dellomonaco, C., Clomburg J.M., Miller E.N., and Gonzalez, R. (2011) Engineered reversal of the B-oxidation cycle for the synthesis of fuels and chemicals. Nature, 476:355-360.
 
Murarka, A., Clomburg, J. M., Moran, S., Shanks, J.V., and Gonzalez, R. (2010). Metabolic analysis of wild-type Escherichia coli and a pyruvate dehydrogenase (PDH)-deficient derivative reveals the role of PDH in the fermentative metabolism of glucose. Journal of Biologial Chemistry, 285 (41): 31548-31558.

Mazumdar, S., Clomburg, J.M., and Gonzalez, R. (2010) Escherichia coli strains engineered for the homofermentative production of D-lactic acid from glycerol. Applied and Environmental Microbiology, 76 (13): 4327-4336.

Blankschien, M., Clomburg, J.M., and Gonzalez, R. (2010) Metabolic Engineering of Escherichia coli for the Production of Succinate from Glycerol. Metabolic Engineering, 12 (5): 409-419.

Murarka, A., Clomburg, J.M., and Gonzalez, R. (2010). Metabolic flux analysis of wild-type Escherichia coli and mutants deficient in pyruvate-dissimilating enzymes during fermentative metabolism of glucuronate. Microbiology-SGM, 156 (6): 1860 - 1872.

Clomburg, J.M., and Gonzalez, R. (2010) Biofuel production in Escherichia coli: The role of metabolic engineering and synthetic biology. Applied Microbiology and Biotechnology, 86 (2): 419-434.

Durnin, G., Clomburg, J.M., Yeates, Z., Alvarez, P.J.J., Zygourakis, K., Campbell, P., and Gonzalez, R. (2009). Understanding and Harnessing the Microaerobic Metabolism of Glycerol in Escherichia coli. Biotechnology and Bioengineering 103 (1): 148-161.


Contact Information

Abercrombie Lab, C124
Rice University
6100 Main Street MS-362
Houston, TX, 77005
Fax (713) 348-5478
jmclomburg@rice.edu