Dicty set-up protocols

Plate recipes	PDF	DOC
Freezing dicty clones	PDF	DOC
Making bacteria	PDF	DOC
Plating from frozen spores	PDF	DOC
Counting cells Dicty field collection	PDF	DOC
Culturing clones from soil	PDF	DOC
Master sheet for freezing Dicty collections		XLS

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Dicty experimental protocols

Setting up mixing experiments	PDF	DOC
Slug racing using spores	PDF	DOC
REMI mutagenesis	PDF	DOC
Plasmid cloning from Dicty following REMI	PDF	DOC
CellTracker	PDF
Time lapse microscopy on our Nikon	PDF

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Tissue / liquid culture protocols

Setting up tissue culture	PDF
Counting and cutting back cells	PDF	DOC

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DNA / PCR protocols protocols

Extracting DNA from spores	PDF
Extracting DNA from cells	PDF
Designing primers	PDF	DOC
Making primer stocks	PDF	DOC
PCR optimization	PDF	DOC
Ethanol precipitation for genotyping	PDF	DOC

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Mutation accumulation (MA) experiment protocols

Checking MA cell growth	PDF	DOC
MA transfer protocol	PDF	DOC

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