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Seminars
Microfluidic Manipulation of Protein-DNA Interactions
Dr. Ronald G. Larson
Department of Chemical and Biomolecular Engineering
University of Michigan, Ann Arbor, Michigan
When: Thursday, September 6, 2007
Time: 2:30PM - 3:30PM
Where: 1070 Duncan Hall
Abstract:
We develop microfluidic methods for both measuring polymer dynamics in confined spaces, and for manipulating and imaging single biopolymers, including DNA and proteins, using flow fields, electric fields, and surfaces. These methods are of importance both in the development of microfluidic devices for processing of DNA and other large molecules for genomics applications, and to learn how proteins interact with DNA in cellular processes such as DNA repair, transcription, etc. Using single-molecule optical microscopy and atomic force microscopy experiments combined with Brownian dynamics simulations of DNA molecules, and fluid mechanics analyses, we consider isolated and semi-dilute DNA molecules near absorbing and non absorbing walls in the presence of flows. In particular, we explore ways in which DNA unraveling can be optimized through manipulation of flow and electric fields, and how one can design theoretical models to explain the unraveling processes. Applications of these methods to analysis of DNA –protein interactions are discussed.
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